Use of anionic denaturing detergents to purify insoluble proteins after overexpression
نویسندگان
چکیده
منابع مشابه
Use of anionic denaturing detergents to purify insoluble proteins after overexpression
BACKGROUND Many proteins form insoluble protein aggregates, called "inclusion bodies", when overexpressed in E. coli. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chaotropic agents such as urea or guanidinium hydrochloride had been shown, these compounds were predominantly used to dissolve inclusion bodies. Other denaturants exist but have...
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The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time consuming. This review presents an overview of methods for purification o...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2012
ISSN: 1472-6750
DOI: 10.1186/1472-6750-12-95